Prosomeric Hypothalamic Distribution of Tyrosine Hydroxylase Positive Cells in Adolescent Rats

This study was funded by the Spanish Ministry of Science, Innovation, and Universities (MCIU), State Research Agency (AEI), and European Regional Development Fund (FEDER); PGC2018-098229-B-100 to JF and by Seneca Foundation (19904/GERM/15).Most of the studies on neurochemical mapping, connectivity, and physiology in the hypothalamic region were carried out in rats and under the columnar morphologic paradigm. According to the columnar model, the entire hypothalamic region lies ventrally within the diencephalon, which includes preoptic, anterior, tuberal, and mamillary anteroposterior regions, and sometimes identifying dorsal, intermediate, and ventral hypothalamic partitions. This model is weak in providing little or no experimentally corroborated causal explanation of such subdivisions. In contrast, the modern prosomeric model uses different axial assumptions based on the parallel courses of the brain floor, alar-basal boundary, and brain roof (all causally explained). This model also postulates that the hypothalamus and telencephalon jointly form the secondary prosencephalon, separately from and rostral to the diencephalon proper. The hypothalamus is divided into two neuromeric (transverse) parts called peduncular and terminal hypothalamus (PHy and THy). The classic anteroposterior (AP) divisions of the columnar hypothalamus are rather seen as dorsoventral subdivisions of the hypothalamic alar and basal plates. In this study, we offered a prosomeric immunohistochemical mapping in the rat of hypothalamic cells expressing tyrosine hydroxylase (TH), which is the enzyme that catalyzes the conversion of L-tyrosine to levodopa (L-DOPA) and a precursor of dopamine. This mapping was also combined with markers for diverse hypothalamic nuclei [agouti-related peptide (Agrp), arginine vasopressin (Avp), cocaine and amphetamine-regulated transcript (Cart), corticotropin releasing Hormone (Crh), melanin concentrating hormone (Mch), neuropeptide Y (Npy), oxytocin/neurophysin I (Oxt), proopiomelanocortin (Pomc), somatostatin (Sst), tyrosine hidroxilase (Th), and thyrotropin releasing hormone (Trh)]. TH-positive cells are particularly abundant within the periventricular stratum of the paraventricular and subparaventricular alar domains. In the tuberal region, most labeled cells are found in the acroterminal arcuate nucleus and in the terminal periventricular stratum. The dorsal retrotuberal region (PHy) contains the A13 cell group of TH-positive cells. In addition, some TH cells appear in the perimamillary and retromamillary regions. The prosomeric model proved useful for determining the precise location of TH-positive cells relative to possible origins of morphogenetic signals, thus aiding potential causal explanation of position-related specification of this hypothalamic cell type.State Research Agency (AEI)European Commission PGC2018-098229-B-100Fundacion Seneca 19904/GERM/15Spanish Governmen

STUDY OF THE MINIMUM SPOUTING VELOCITY IN A DRAFT TUBE CONICAL SPOUTED BED

A study has been carried out on the hydrodynamics of conical spouted beds with a non-porous draft-tube. A hydrodynamic correlation has been proposed for calculating the minimum spouting velocity as a function of dimensionless moduli that take into account geometric factors, particle characteristics and operating conditions. A statistical analysis of the data obtained following a design of experiments shows that the inlet diameter, particle diameter and the height of the entrainment zone are the parameter of greater influence on the minimum spouting velocity

Chileans facing immigration: Relations between orientations of acculturation, perception of threat and social well-being in the great concepcion

Indexación: Scopus; Scielo; Redalyc.En Chile, el aumento sostenido de la inmigración extranjera ha impactado en la construcción de nuevas formas de relación social. Si bien los procesos aculturativos han sido estudiados desde la perspectiva de los inmigrantes por numerosas investigaciones, los miembros de las sociedades receptoras han recibido menor atención. Este trabajo tiene como propósito identificar las preferencias de aculturación de N=553 chilenos, y verificar sus relaciones con la percepción de amenaza y el bienestar social. Contrario a lo esperado, el individualismo aculturativo se asoció con un menor grado de amenaza percibida y una mejor evaluación del propio funcionamiento social que el integracionismo. Estos hallazgos son relacionados con la incorporación de modelos de ciudadanía promovidos por la ideología neoliberal en el Chile post-dictatorial.In Chile, the sustained increase of migratory flows is producing an important impact on building new forms of social relations. Acculturation processes have been studied from immigrant’s perspective by numerous studies, but host majority members has received less attention from scholars. is study aimed to verify relations between acculturation preferences of N=553 Chilean participants, their social well-being and perceived threat. Results showed that, contrary as expected, individualism was positively linked with better social functioning and less perceived threat than integrationism, suggesting the impact of neoliberal ideology on post-dictatorial Chilean culture.http://www.redalyc.org/jatsRepo/647/64753989005/index.htm

New high resolution calcareous nannofossil analysis across the Danian / Selandian transition at the Zumaia section: comparison with South Tethys and Danish sections

A high resolution calcareous nannofossil biostratigraphic analysis was carried out across the Danian/Selandian transition at the Zumaia section (western Pyrenees). In the studied interval, spanning Varol’s Subzone NTp7a to Zone NTp9, a sequence of 10 distinct calcareous nannofossil events is identified. The recognition of these bioevents confirms the continuous and expanded character of the Zumaia section and allows an accurate biostratigraphic correlation of the D/S transition between Zumaia, the Danish reference sections and the more southerly Tethys sections. According to this correlation, the abrupt transition from the upper Danian limestones (the calcisiltite unit) to the Lellinge Greensand that marks the D/S boundary in the type area correlates with the lithological change from the Aitzgorri Limestone Formation to the Itzurun Formation in Zumaia. The calcareous nannofossil bioevents recorded in connection with the organic-rich layer used to mark the base of the Selandian in the Tethys region were detected in Zumaia ~10 m below the top of the Aitzgorri Limestone Formation. This finding suggests that the organic-rich layer is considerably older than the Danian/Selandian (D/S) boundary at Zumaia and the type area, and thus using it to mark the D/S boundary in Tethys is not appropriate. According to a counting of limestone/marl couplets, which are demonstrated to be the stratigraphic expression of precession cycles throughout the Zumaia section, the organic-rich layer in the Tethys region is ~546 kyr older than the D/S boundary at the type area

Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds

Group A rotavirus (RVA) is one of the main causes of neonatal calf diarrhea worldwide. RVA strains affecting Argentinean cattle mainly possess combinations of the G6, G10, P[5] and P[11] genotypes. To determine RVA diversity among Argentinean cattle, representative bovine RVA strains detected in diarrheic calves were selected from a survey conducted during 1997–2009. The survey covered the main livestock regions of the country from dairy and beef herds. Different phylogenetic approaches were used to investigate the genetic evolution of RVA strains belonging to the prevalent genotypes. The nucleotide phylogenetic tree showed that all genotypes studied could be divided into several lineages. Argentinean bovine RVA strains were distributed across multiple lineages and most of them were distinct from the lineage containing the vaccine strains. Only the aminoacid phylogenetic tree of G6 RVA strains maintained the same lineages as observed at the nucleotide level, whereas a different clustering pattern was observed for the aminoacid phylogenetic trees of G10, P[5] and P[11] suggesting that the strains are more closely related at the aminoacid level than G6 strains. Association between P[5] and G6(IV), prevalent in beef herd, and between P[11] and G6(III) or G10 (VI and V), prevalent in dairy herds, were found. In addition, Argentinean G6(III), G10, P[5] and P[11] bovine RVA strains grouped together with human strains, highlighting their potential for zoonotic transmission. Phylogenetic studies of RVA circulating in animals raised for consumption and in close contact with humans, such as cattle, contribute to a better understanding of the epidemiology of the RVA infection and evolution.Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matthijnssens, J.. University of Leuven. Rega Institute for Medical Research; BélgicaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; ArgentinaFil: Bilbao, Gladys Noemí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Fernandez, Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Parra, G. I.. National Institutes of Health; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Network Coding-Based Next-Generation IoT for Industry 4.0

Industry 4.0 has become the main source of applications of the Internet of Things (IoT), which is generating new business opportunities. The use of cloud computing and artificial intelligence is also showing remarkable improvements in industrial operation, saving millions of dollars to manufacturers. The need for time-critical decision-making is evidencing a trade-off between latency and computation, urging Industrial IoT (IIoT) deployments to integrate fog nodes to perform early analytics. In this chapter, we review next-generation IIoT architectures, which aim to meet the requirements of industrial applications, such as low-latency and highly reliable communications. These architectures can be divided into IoT node, fog, and multicloud layers. We describe these three layers and compare their characteristics, providing also different use-cases of IIoT architectures. We introduce network coding (NC) as a solution to meet some of the requirements of next-generation communications. We review a variety of its approaches as well as different scenarios that improve their performance and reliability thanks to this technique. Then, we describe the communication process across the different levels of the architecture based on NC-based state-of-the-art works. Finally, we summarize the benefits and open challenges of combining IIoT architectures together with NC techniques

Experimental Study of the Pyrolysis of NH3under Flow Reactor Conditions

The possibility of using ammonia (NH3), as a fuel and as an energy carrier with low pollutant emissions, can contribute to the transition to a low-carbon economy. To use ammonia as fuel, knowledge about the NH3 conversion is desired. In particular, the conversion of ammonia under pyrolysis conditions could be determinant in the description of its combustion mechanism. In this work, pyrolysis experiments of ammonia have been performed in both a quartz tubular flow reactor (900-1500 K) and a non-porous alumina tubular flow reactor (900-1800 K) using Ar or N2 as bath gas. An experimental study of the influence of the reactor material (quartz or alumina), the bulk gas (N2 or Ar), the ammonia inlet concentration (1000 and 10a 000 ppm), and the gas residence time [2060/T (K)-8239/T (K) s] on the pyrolysis process has been performed. After the reaction, the resulting compounds (NH3, H2, and N2) are analyzed in a gas chromatograph/thermal conductivity detector chromatograph and an infrared continuous analyzer. Results show that H2 and N2 are the main products of the thermal decomposition of ammonia. Under the conditions of the present work, differences between working in a quartz or non-porous alumina reactor are not significant under pyrolysis conditions for temperatures lower than 1400 K. Neither the bath gas nor the ammonia inlet concentration influence the ammonia conversion values. For a given temperature and under all conditions studied, conversion of ammonia increases with an increasing gas residence time, which results into a narrower temperature window for NH3 conversion

Velocity shear instability and plasma billows at the Earth\u27s magnetic boundary

The Kelvin-Helmoltz instability (KH) with formation of vortices appears in a wide variety of terrestrial, interplanetary, and astrophysical contexts. We study a series of iterated rolled-up coherent plasma structures (15) that flow in the equatorial Earth\u27s boundary layer (BL), observed on October 24, 2001. The data were recorded during a 1.5 hour-long Wind crossing of the BL at the dawn magnetospheric flank, tailward of the terminator (X≈−13 RE). The interplanetary magnetic field (IMF) was radially directed, almost antiparallel to the magnetosheath (MS) flow. This configuration is expected to be adverse to the KH instability because of the collinearity of field and flow, and the high compressibility of the MS. We analyze the BL stability with compressible MHD theory using continuous profiles for the physical quantities. Upstream, at near Earth sites, we input parameters derived from an exact MHD solution for collinear flows. Further downtail at Wind position we input measured parameters. The BL is found KH unstable in spite of unfavorable features of the external flow. On the experimental side, the passage of vortices is inferred from the presence of low density - hot plasma being accelerated to speeds higher than that of the contiguous MS. It is further supported by the peculiar correlation of relative motions (in the bulk velocity frame): cold-dense plasma drifts sunward, while hot-tenuous plasma moves tailward. This event differs from many other studies that reported BL vortices under strongly northward IMF orientations. This is a case of KH vortices observed under an almost radial IMF, with implicit significance for the more common Parker\u27s spiral fields, and the problem of plasma entry in the magnetosphere

Chronic Neuropsychological Sequelae of Cholinesterase Inhibitors in the Absence of Structural Brain Damage: Two Cases of Acute Poisoning

Here we describe two cases of carbamate poisoning. Patients AMF and PVM were accidentally poisoned by cholinesterase inhibitors. The medical diagnosis in both cases was overcholinergic syndrome, as demonstrated by exposure to cholinesterase inhibitors. The widespread use of cholinesterase inhibitors, especially as pesticides, produces a great number of human poisoning events annually. The main known neurotoxic effect of these substances is cholinesterase inhibition, which causes cholinergic overstimulation. Once AMF and PVM had recovered from acute intoxication, they were subjected to extensive neuropsychological evaluation 3 and 12 months after the poisoning event. These assessments point to a cognitive deficit in attention, memory, perceptual, and motor domains 3 months after intoxication. One year later these sequelae remained, even though the brain magnetic resonance imaging (MRI) and computed tomography (CT) scans were interpreted as being within normal limits. We present these cases as examples of neuropsychological profiles of long-term sequelae related to acute poisoning by cholinesterase inhibitor pesticides and show the usefulness of neuropsychological assessment in detecting central nervous system dysfunction in the absence of biochemical or structural markers

On the combination of multi-cloud and network coding for cost-efficient storage in industrial applications

The adoption of both Cyber–Physical Systems (CPSs) and the Internet-of-Things (IoT) has enabled the evolution towards the so-called Industry 4.0. These technologies, together with cloud computing and artificial intelligence, foster new business opportunities. Besides, several industrial applications need immediate decision making and fog computing is emerging as a promising solution to address such requirement. In order to achieve a cost-efficient system, we propose taking advantage from spot instances, a new service offered by cloud providers, which provide resources at lower prices. The main downside of these instances is that they do not ensure service continuity and they might suffer from interruptions. An architecture that combines fog and multi-cloud deployments along with Network Coding (NC) techniques, guarantees the needed fault-tolerance for the cloud environment, and also reduces the required amount of redundant data to provide reliable services. In this paper we analyze how NC can actually help to reduce the storage cost and improve the resource efficiency for industrial applications, based on a multi-cloud infrastructure. The cost analysis has been carried out using both real AWS EC2 spot instance prices and, to complement them, prices obtained from a model based on a finite Markov chain, derived from real measurements. We have analyzed the overall system cost, depending on different parameters, showing that configurations that seek to minimize the storage yield a higher cost reduction, due to the strong impact of storage cost